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CIDE-B (Cell Death Inducing DFFA-like Effector B, Cell Death Activator CIDE-B, CIDEB)

CIDE-B (Cell Death Inducing DFFA-like Effector B, Cell Death Activator CIDE-B, CIDEB)

Cat no: C5072-51E

Supplier: United States Biological
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Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified (1). CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD (1,2). Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes, and bone marrow (1). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Murine liver tissue lysate. ~25kDa Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5072-51E
Reactivities: Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.2% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by ammonium sulfate precipitation.
References: 1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33 2. Inohara N, Koseki T, Chen S, Benedict MA, Nunez G. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4
Additional info: Recognizes mouse CIDE-B.

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