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CLTA, Recombinant, Human, aa1-218, His-Tag (Clathrin Light Chain A, Lca)

Cat no: 045416


Supplier: United States Biological
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Clathrin, light chain A, also known as CLTA, belongs to the clathrin family. Clathrin is a large, soluble protein composed of heavy and light chains. It is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are captured by Clathrin-coated vesicles. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements. Source: Recombinant corresponding to aa1-218 from human CLTA, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~26.2kD (242aa) confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAELDP FGAPAGAPGG PALGNGVAGA GEEDPAAAFL AQQESEIAGI ENDEAFAILD GGAPGPQPHG EPPGGPDAVD GVMNGEYYQE SNGPTDSYAA ISQVDRLQSE PESIRKWREE QMERLEALDA NSRKQEAEWK EKAIKELEEW YARQDEQLQK TKANNRAAEE AFVNDIDESS PGTEWERVAR LCDFNPKSSK QAKDVSRMRS VLISLKQAPL VH Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 045416
Size: 100ug
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 10% glycerol.
Purity: ~90% (SDS-PAGE)
References: 1. Kirchhausen T., et al. (1987) Proc Natl Acad Sci USA. 84:8805-8809. 2. Liu S H., et al. (1995) Cell. 83: 257-267.

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