Coenzyme A Assay Kit

Coenzyme A (CoA) functions as an acyl group carrier and carbonyl-activating group in a number of key biochemical reactions, including the TCA cycle and fatty acid metabolism. CoA is involved in over 100 different reactions in intermediary metabolism with approximately 4% of known enzymes utilizing CoA as a cofactor. Cayman's Coenzyme A Assay Kit can be used for assaying coenzyme A from plasma, serum, urine, cell lysates, and tissue homogenates. In this assay, CoA forms a complex with europium chloride and tetracycline in the presence of periodic acid. Fluorescence intensity of this complex is analyzed with an excitation wavelength between 380-390 nm and an emission wavelength between 610-615 nm.

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SPECIFICATIONS

Catalog Number

700440

Form

96 Well

P Type

Assay Kits|Small Molecule Detection (Non-EIA)

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Coenzyme A (CoA) is an indispensible cofactor in all living organisms, functioning as an acyl group carrier and carbonyl-activating group in a number of key biochemical reactions, including the TCA cycle and fatty acid metabolism. CoA is involved in over 100 different reactions in intermediary metabolism with approximately 4% of known enzymes utilizing CoA as a cofactor. Cayman's Coenzyme A Assay Kit provides a simple, reproducible, and sensitive tool for assaying coenzyme A from plasma, serum, urine, cell lysates, and tissue homogenates. In this assay, CoA forms a complex with europium chloride and tetracycline in the presence of periodic acid. Fluorescence intensity of this complex is analyzed with an excitation wavelength between 380-390 nm and an emission wavelength between 610-615 nm.