Collagen Type II (BSA & Azide Free)

Applications:\nSuitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1-2ug/ml for 2hrs at RT.\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37 degrees C.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nCartilage in lung or fetus.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-18ZX

Size

100ul

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-18Z.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Mayne, R., et al., Conn. Tissue Res., 31(1): 11-21 (1994). 2. Chen, Q., et al., Dev Dynamics 196(1): 47- 53 (1993). 3. Hicks, D.G., et al., J. Histotechnology 20: 215 (1997). 4. PNAS 91: 5070-5074 (1994). 5. Exp. Cell Res. 219: 257-265 (1995). 6. Cell 80: 423-430 (1995). 7. Develop. Dynamics 200: 294-304 (1994). 8. J. Cell Biol. 130: 1461-1472 (1995). 9. Develop. Biol. 170: 387-396 (1995).

Additional Info

Highly specific to type II collagen and lathyritic type II collagen. Does not crossreact with types I, III, IV, V, VI, IX, X, or XI. In Western Blot, recognizes TCB fragment of pepsin-digested type II collagen after digestion with mammalian collagenase. Species Crossreactivity: human, mouse, rat, bovine, chicken.