Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

The collagen family is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral selfassociation in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function, such as bone strength and platelet activation.\n\nApplications: \nSuitable for use in ELISA, Dot and Slot Blot, Indirect Immunofluorescence and Indirect Immunohistochemistry. Not recommended on Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA: 1:3000\nImmunofluorescence (Indirect): 1:20-1:40\nImmunohistochemistry (Indirect): 1:20-1:40. Use frozen or acetone fixed specimens (paraffin not recommended)\nDot/Slot Blot: 1:300\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7510-50E

Size

50ug

Applications

ELISA, IF, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, mannitol, dextran. No preservatives added.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1.Rennekampff, H.O. et al. (1996). Surgery. 120(1):16

Additional Info

Recognizes native and heat denatured human Collagen Type IV. Antiserum was passed over immobilized human blood plasma proteins and human collagen types I, III and V. Human plasma does not interfere with binding to collagen. Crossreactivity: collagen types I, III and V; <10%; human collagen II; <1%.