COL13A1 (collagen 13alpha1) is a 95kD member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfidelinked
homotrimers and participates in cell adhesion by binding to the integrin a1 subunit, nidogen2,
and fibronectin. Human COL13A1 is 717aa in length. It has an Nterminal 44aa cytoplasmic region plus a 656aa extracellular domain (ECD) (aa62-717).The ECD contaiNeat serum four non collagenous Neat serum (NC) regions (aa1-121,217-269,442-463, 700-717)
interspersed among three collagenous (COL) domains (aa122-216, 270-441,464-699). Multiple splice forms exist and typically involve deletions of 12-30aa between aa220-705. Proteolytic cleavage after Arg108 generates an 8590kD soluble form. Over aa109-668 (based on a human isoform that shows a deletion of aa239-260, 551-565, 616-627),human COL13A1 shares 91% aa sequence identity with mouse COL13A1.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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