Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Applications:
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
immunohistochemistry (Frozen): 1:10-1:40
ELISA: 1:500 -1:2000
Immunofluorescence: 1:10-1:40
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.