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Colorimetric COX (ovine) Inhibitor Screening Assay Kit

Cat no: 760111

Colorimetric COX (ovine) Inhibitor Screening Assay Kit

The Colorimetric COX Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm. Inhibition of COX activity by a variety of selective and nonselective inhibitors shows potencies similar to those observed with other in vitro methods. The Colorimetric COX Inhibitor Screening assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors.

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SPECIFICATIONS

Catalog Number

760111

Form

96 Well

P Type

Assay Kits|Eicosanoids

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

The Colorimetric COX Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The Colorimetric COX Inhibitor Screening assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. The Cayman Colorimetric COX Assay is a time saving tool for screening vast numbers of inhibitors.

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