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Complement C1q, Human (Complement Component 1, q Subcomponent)

Cat no: C0010-02H

Complement C1q, Human (Complement Component 1, q Subcomponent)

C1q is the first component of the classical pathway of complement activation. C1q along with the enzymatically active components C1r and C1s forms the C1 complex. When C1 binds to immunoglobulins in the form of immune complexes, it leads to activation of C1r and C1s proteases and a further activates the classical pathway of complement. C1q is a glycoprotein that belongs to the collectin family, having a molecular weight of about 410-462kD. C1q is a hexamer composed of globular heads attached to collagen-like triple-helix tails. The globular heads of C1q exclusively bind to the CH2 domain of IgG molecules or the CH3 domain of IgM. Each heavy chain of the immunoglobulin molecule contains a single binding site for C1q. Given that C1q must bind to no less than two heavy chains in order to alter its conformation and activate C1r and C1s, its activation follows only after binding to immunoglobulins in the form of immune complexes bound to multivalent antigens. C1q

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SPECIFICATIONS

Catalog Number

C0010-02H

Size

1mg

Applications

ELISA

Form

Supplied as a lyophilized powder from 0.01M Na2EDTA, 0.3M sodium chloride, pH 7.5. Preservative free. Reconstitute with sterile dH2O to 1ml.

Purity

~96% (SDS PAGE)

References

1. Wenderfer S. E. et al., Arthritis Res Ther 9:R114 (2007) 2. Kanda Y et al. Erratum in Glycobiology 17:1030 (2007)

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