Home  >  Products  >  Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (HRP)
Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (HRP)

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (HRP)

Cat no: C7850-14U4

Supplier: United States Biological
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C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Peroxidase/IgG protein molar ratio (E/P): ~1.8 Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Catalogue number: C7850-14U4
Reactivities: Rat
Hosts: Goat
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Horseradish Peroxidase (HRP). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Purified
Additional info: Recognizes rat C3, C3c. In immunoelectrophoresis against fresh rat serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of rat serum or plasma.

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