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Complement C9

Cat no: C7850-52

Complement C9

Complement factor C9 is a 71kD single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and have an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). Primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60ug/ml, and up-regulated from hepatocytes by IFN-g.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:30,000 Recognizes C9 in human seum diluted 1:50 in Tris buffer (20mM Tris-base, 1mM magnesium chloride, 1mM calcium chloride, 140mM sodium chloride) and incubated for 2 hours at 37C using a human IgM coated (10ug/ml overnight at 4C, blocked with PBS, pH 7.2, 1% BSA for 1 hour).\nOptimal dilutions to be determined by the researcher.\n\nCulture Medium: \nRPMI 1640 with 2-10% fetal calf serum\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7850-52

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1,k

References

The Complement Facts Book. 2000. Bernard J. Morlay and Mark J. Walport (eds.). Academic Press, London.

Additional Info

Recognizes human complement C9.

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