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Complement C9 (Complement Component 9)

Complement C9 (Complement Component 9)

Cat no: C7850-51F

Supplier: United States Biological
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Complement component C9 (CCC9) is part of the mem- brane attack complex and binds to the C5b-8 structure that allows a conformational change to occur enabling CCC9 to insert into the cell to form transmembrane channels. In this manner, it acts similarly to perforin. This antibody is particularly useful in identifying regions of recent ischemic myocardial damage in postmortem myocardum. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:50-1:100 for 1 hr. at RT Optimal dilutions to be determined by the researcher. Positive Control: Myocardium (diffuse cytoplasmic staining of necrotic areas) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7850-51F
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG1
Purity: Supernatant
References: 1. Ferreira M A S, Owen H E and Howie A J. High prevalence of acute myocardial damage in a hospital necropsy series, shown by C9 immunohistology. J. Clin. Pathol. 51:548-551 (1998). 2. Ratnoff W D, Brockman W W and Hasty L A. Immunohistochemical localization of C9 neoantigen and the terminal complement inhibitory protein CD59 in human endometrium. American Journal of Reproductive Immunology. 34:72-79 (1995). 3. Stanley K K, Kocher h-P, Luzio P J, et al.. The sequence and topology of human complement component C9. The EMBO Journal. 4(2):375-382 (1985).
Additional info: Recognizes Complement Component C9. Species Crossreactivity: Human.

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