COMT, NT (COMT, Catechol O-methyltransferase)

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.\n\nApplications:\nSuitable for use in Western Blot, ELISA.\n\nRecommended Dilution: \nELISA: 1:1,000\nWestern Blot: 1:100~400\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

030887

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, crude ascites with 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Paloyelis, Y., et al. Neuropsychopharmacology 35(12):2414-2426(2010)\nStroth, S., et al. Neurobiol Learn Mem 94(3):364-372(2010)\nLim, J.H., et al. Pharmacogenet. Genomics 20(10):605-610(2010)\nDemetrovics, Z., et al. Compr Psychiatry 51(5):510-515(2010)\nBodenmann, S., et al. Sleep 33(8):1027-1035(2010)

Additional Info

Human

Alternative Names

COMT, Catechol O-methyltransferase