Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)

Connexin 31 (Cx31) is a 270 amino acid gap junction protein in mouse and rat with a predicted molecular weight of ~31kD.1 Connexin 31 is encoded by the gene Gjb3, and is expressed in a complex pattern throughout mouse development. During early mouse development, Cx31 is present in both the inner cell mass and the trophectoderm, but it is restricted to cells derived from the trophectoderm lineage after implantation. In adult female rodents, Cx31 is expressed in the granular layer of the placenta, and the lack of Cx31 leads to transient dysmorphogenesis affecting embryonic survival. In adult male rodents, Cx31 is expressed in the seminiferous epithelium of testis and may play a role in cell-cell communication during spermatogenensis.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1-3ug/ml\nWestern Blot: 1-3ug/ml\nImmunohistochemistry: 1-3ug/ml on fixed and paraformaldehyde-perfued tissues\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7853-06

Size

50ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by Immunoaffinity chromatgraphy.

Isotype

IgG

References

1. Hennemann, et al. Eur J Cell Biol 57:51-58, 1992. 2. Dahl, et al. J Cell Sci 109(Pt 1):191-197, 1996. 3. Plum, et al. Dev Biol 231:334-347, 2001. 4. Mok, et al. FEBS Lett 453:243-248, 1999.

Additional Info

Recognizes the ~32kD mouse connexin 31 on Western Blots. The band observed at ~62kD is probably due to dimerization of connexin 31. Several higher bands of unknown origin are observed in Western Blots of mouse skin homogenates. Reactivity has been confirmed with mouse skin homogenate.