Contactin 2 (Transient Axonal Glycoprotein 1, TAG1, CNTN2)

Contactin-2, also known as transient axonal glycoprotein 1 (TAG-1), is a 135kD GPI-anchored protein that is expressed on neurons and glial cells. The extracellular domain of contactin-2 consists of six Ig-like C2-set domains followed by four fibronectin type III domains. Contactin-2 is required for axonal migration and interacts homophilically at juxtaparanodal regions on myelinated fibers. Within the ECD, human Contactin-2 shares 93% aa sequence identity with mouse and rat Contactin-2.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Contactin-2 (rhCNTN2; aa 29-1012: Accession # NP_005067).\n\nApplications: \nWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human CNTN2. Using a colorimetric detection system, the detection limit for rhCNTN2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with will increase sensitivity by 5 to 50 fold. In this application, the use of human CNTN2 monoclonal antibody is recommended.\nImmunohistochemistry: This antibody was used at a concentration of 25ug/ml with appropriate secondary reagents and basic antigen retrieval to detect CNTN2 in paraffin- embedded normal human hippocampus tissue sections. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CNTN2. The detection limit for rhCNTN2 is approximately 10 ng/well. Optimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7865-21

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Selected for its ability to detect human CNTN2 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhCNTN4, rhCNTN5, or rmCNTN3.