Cortisol, Human, BioAssay(TM) ELISA Kit

The United States Biological Cortisol, Human, BioAssay(TM) ELISA Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol concentration in serum and plasma.\n\nCortisol is a steroid hormone released from the adrenal cortex in response to a hormone called ACTH (produced by the pituitary gland). It is involved in the response to stress; it increases blood pressure and blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. In plasma, cortisol is bound to corticosteroid-binding globulin (CBG, transcotin) with high affinity, and to albumin. Only free cortisol is available to most receptors. The amount of cortisol present in the serum undergoes diurnal variation, with the highest levels present in the early morning, and lower levels in the evening, several hours after the onset of sleep. Highest levels are at about 6-8 a.m. and lowest levels are at about midnight. These normal endogenous functions are the basis for the physiological consequences of chronic stress-prolonged cortisol secretion causes muscle wastage, hyperglycemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs. \n\nPrinciple of the Assay:\nCortisol (antigen) in the sample competes with enzyme-labeled cortisol HRP-cortisol) for binding onto limited antibody binding sites on the anti-cortisol antibody immobilized on the surface of the microtiter plate. After incubation, bound and free cortisol are separated by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB substrate are then added. After an appropriate amount of time has elapsed for maximum color development, the enzyme reaction is stopped and the absorbance determined. The concentration of cortisol present in the sample is calculated based on a calibration curve constructed from a series of standards. The color intensity is inversely proportional to the cortisol concentration in the sample.\n\nKit Components:\nMicrotiter Plate, 1x96 wells.\nCortisol Standard, 0ng/ml, 1x1ml \nCortisol Standard, 10ng/ml, 1x1ml \nCortisol Standard, 50ng/ml, 1x1ml \nCortisol Standard, 150ng/ml, 1x1ml \nCortisol Standard, 500ng/ml, 1x1ml \nHRP-Cortisol, 1x21ml\nTMB Substrate, 1x12ml\nStop Solution, 1x12ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7904-20B

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Foster, L. B. and Dunn, R.T. Clin. Chem: 20/3, 365 (1974) 2. De Lacerda, L., Kowarski, A., and Migeon, C.J. J. Clin. Endocr. and Metab: 36: 227 (1973) 3. Rolleri, E.,et al., Clin chim Acta 66: 319 (1976) 4. Kobayashi, Y., et al Steroids, 32 no. 1 (1978) 5. Arakawa, H., Maeda, M., Tsuji, A. Anal. Biochem. 97: 248 (1979).