Creatine Kinase Fluorometric Assay Kit

CK catalyzes the reversible phosphorylation of creatine by ATP to form ADP and phosphocreatine, the major storage form of high energy phosphate in muscle. CK (in blood) is assayed as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, acute renal failure, and additional complications. Cayman's Creatine Kinase Fluorometric Assay provides a convenient method for detecting total CK activity in plasma and serum. CK catalyzes the reversible reaction between creatine phosphate and ADP to form creatine and ATP. Through a series of enzyme-coupled reactions, formaldehyde is generated. The cyclization of formaldehyde and acetoacetanilide in the presence of ammonia results in a fluorescent product which is analyzed using an excitation wavelength of 365-375 nm and an emission wavelength of 465-475 nm.

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SPECIFICATIONS

Catalog Number

700630

Form

96 Well

P Type

Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Creatine kinase (CK) catalyzes the reversible phosphorylation of creatine by ATP to form ADP and phosphocreatine, the major storage form of high energy phosphate in muscle. CK (in blood) is assayed as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, and acute renal failure. There is an inverse relationship in the serum levels of T3 and CK in thyroid disease. Lowered CK activity can also be an indication of alcoholic liver disease and rheumatoid arthritis. Cayman's Creatine Kinase Assay provides a convenient method for detecting total CK activity in plasma and serum. CK catalyzes the reversible reaction between creatine phosphate and ADP to form creatine and ATP. Through a series of enzyme-coupled reactions, formaldehyde is generated. The cyclization of formaldehyde and acetoacetanilide in the presence of ammonia results in a fluorescent product which is analyzed using an excitation wavelength of 365-375 nm and an emission wavelength of 465-475 nm.