Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate for the production of ATP. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. Under normal conditions, its formation occurs at a rate that is relatively constant and so creatinine is a useful tool for normalizing the levels of other molecules found in urine.
A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve. Standards or samples are pipetted into the clear microtiter plate. The color generating reaction is initiated with the Creatinine Reagent added to all standards, controls and samples. After a short incubation the intensity of the generated color is read in a microtiter plate reader at 490nm. This assay has been validated for diluted human urine samples. The end user should evaluate recoveries of creatinine in other species being studied. A NIST creatinine standard, is provided to generate a standard curve. Standards or samples are pipetted into the clear microtiter plate. The color generating reaction is initiated with the Creatinine Reagent added to all standards, controls and samples. After a short incubation the intensity of the generated color is read in a microtiter plate reader at 490nm.
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