Crk p38 (Adapter molecule crk, Avian sarcoma virus CT10 (v crk) oncogene homolog, CRK isoform 2, CRK isoform II, CRKII, p38Proto oncogene C crk, v crk avian, Sarcoma virus CT10 oncogene homolog, v crk Sarcoma virus CT10 oncogene homolog, v crk Sarcoma vir

Crk (p38) are adaptor proteins, composed mostly of SH2 and SH3 domains, which play a key role in cellular signaling by mediating the construction of protein complexes and also by managing cell proliferation, differentiation, and migration (1). They are critical integrators \nof upstream signals for cell invasion and migration in human cancer cell lines. Crk is believed to be a regulator of invasive responses because increased levels of the protein have been \nobserved in multiple human cancers (2). In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth (3). Crk \nassociates with ERM family proteins (including ezrin, radixin, and moesin), activates RhoA, and promotes cell motility toward hyaluronic acid (4). \n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000 \nImmunocytochemistry: 1:250-500 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C7924-10B

Size

100ul

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Nishihara H, et al. Cancer Lett (1):55-61, 2002. 2. Watanabe T, et al. Mol Cancer Res. (7):499-510, 2006. 3. Rodrigues SP, et al. Mol Cancer Res. (4):183-94, 2005. 4. Tsuda MJ, et al. Biol Chem. (45):46843-50, 2004.

Additional Info

Recognizes human Crk/p38 protein.