CTH (Cysteine-protein sulfhydrase, Gamma-cystathionase)

CTH(CYSTATHIONINE GAMMA-LYASE),also called CYSTATHIONASE, is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate.And the CTH gene is mapped on 1p31.1. In some bacteria and mammals, including humans, this enzyme takes part in generating hydrogen sulfide and that genetic deletion of this enzyme in mice markedly reduced H2S levels in the serum, heart, aorta, and other tissues. Mutant mice lacking CTH displayed pronounced hypertension and diminished endothelium-dependent vasorelaxation. Cystathionine was physiologically activated by calcium and calmodulin, which is a mechanism for H2S formation in response to vascular activation.\n\nUniProt Number:\nP32929\n\nGene ID:\nCTH\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144397

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Donald, L. J., Wang, H. S., Hamerton, J. L. Assignment of the gene for cystathionase (CYS) to human chromosome 16.\n2. Ishii, I., Akahoshi, N., Yamada, H., Nakano, S., Izumi, T., Suematsu, M. Cystathionine gamma-Lyase-deficient mice require dietary cysteine to protect against acute lethal myopathy and oxidative injury. J. Biol. Chem. 285: 26358-26368, 2010.\n3. Lu, Y., O'Dowd, B. F., Orrego, H., Israel, Y. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase. Biochem. Biophys. Res. Commun. 189: 749-758, 1992. \n

Additional Info

Recognizes human, mouse and rat CTH. No crossreactivity with other proteins.