Custom Polyclonal Antibodies, Rabbit, 35 Day

USDA-Registered Polyclonal Production\nUnited States Biological is known for more than the thousands of antibodies offered in our catalog. Our antibodies are produced in state of the art facilities in the US.\n\nWe maintain current USDA certification as a qualified animal research facility, and comply with the Animal Welfare Act. We follow guidelines set forth by the National Institute of Health (NIH) and have established an Institutional Animal Care and Use Committee (IACUC) to review all production protocols prior, during and after all client programs.\n\nAntibody Program:\nA typical standard protocol includes animal purchase, housing and care, all immunizations, bleeds and exsanguinations as per our standard protocol formats. Your project may require different specifications and, therefore, prices will vary. Our costs are quite reasonable. Although we will conduct single animal programs, the additional costs to add a second animal can produce a significantly greater yield and likelihood for success.\n\nStandard 2 Rabbit Protocol:\nStandard protocol pricing includes animal purchase, housing and care up to 49 days, all immunizations, bleeds and exsanguinations as per the following schedule:\n\nPre-Peptide Consultation if necessary\n\nRequired, but not included in price:\nPeptide Production\nPeptide Conjugation\n\nDay 0 Pre-immunization bleed\n Antigen Injection (s.c and i.m; CFA; 200ug)\nDay 14 Antigen Injection (IFA; 100ug)\nDay 28 Antigen Injection (IFA; 100ug)\nDay 35 First Bleed (20-25ml/rabbit)\n ELISA\nDay 42 Antigen Injection (IFA; 100ug)\nDay 49 Final Production Bleed (~40-50ml/rabbit)\n ELISA\n Return of antisera and peptides\n\nContinuation (extra cost):\nDay 63 Antigen Injection (IFA; 100ug)\nDay 77 Third Bleed (20-25ml/rabbit)\n\nAntigen Requirements:\nPer Animal Minimum Recommended\nChickens 250ug 1mg \nRabbits 500ug 2mg \nGoats 2.5mg 5mg\nSheep 2.5mg 5mg\n\nThe amount of antigen required varies from a minimum as shown for the standard protocol to a maximum when additional injections might be needed to boost antibody production or to continue a maintenance program longer than the stated time. Affinity columns will also require additional antigen.\n\nAntibody Quotation Worksheet: \nFor a quotation, please supply the following information:\n\n1. Antigen name or peptide sequence\n\n2. Type of protein\no Natural\no Recombinant\no Synthetic \no Purity ___%\no MW. ___\n\n3. Conjugation\no BSA\no KLH\no Ovalbumin\no Thyroglobulin\n\n4. Number of animals required\n\n5. Immunization protocol\no Customer Supplied\no United States Biological Supplied\n\nImportant Note:\nStandard 35 day projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$195/rabbit/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instructions are properly followed.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

CS010-010

Size

1 Rabbit

References

US Biological application reference: Webb, G.D. et al., (2008) J. Pharmacology and Exp. Pharmacology 324:876-882. 2. Han, M.-S. et al., (2004) Proc. Natl., Acad. Sci. 101: 1093-1098.