CXCL1 (C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity)

CXCL1(Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a \nsmall cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, \nGROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha \n(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on \n4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in \nmelanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and \nhas neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the \nmigration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation,\nwound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor \nmigration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected \nenhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially \nregulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis \nsignature genes identified, several, including CXCL1, were functionally validated.\n\nUniProt Number:\nP09341\n\nGene ID:\nCXCL1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144404

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Anisowicz, A., Zajchowski, D., Stenman, G., Sager, R. Functional diversity of GRO gene expression \nin human fibroblasts and mammary epithelial cells. Proc. Nat. Acad. Sci. 85: 9645-9649, 1988.\n2. Horuk, R., Yansura, D. G., Reilly, D., Spencer, S., Bourell, J., Henzel, W., Rice, G., Unemori, \nE. Purification, receptor binding analysis, and biological characterization of human melanoma \ngrowth stimulating activity (MGSA): evidence for a novel MGSA receptor. J. Biol. Chem. 268: \n541-546, 1993.\n3. O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human \nchromosome 4. Cytogenet. Cell Genet. 84: 39-42, 1999.

Additional Info

Recognizes human CXCL1. No crossreactivity with other proteins.