CXCL5 (ENA-78, SCYB5) BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human CXCL5/ENA-78 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nC-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-g. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 10 pg/ml \n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP42830\n\nEntrez Gene ID:\n6374\n\nKit Components:\n96-well plate pre-coated with anti-CXCL5/ENA-78, Human\nLyophilized recombinant CXCL5/ENA-78, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143798

Size

96Tests

References

1. Chang MS, McNinch J, Basu R, Simonet S (Nov 1994). \"Cloning and characterization of the human neutrophil-activating peptide (ENA-78) gene\". J Biol Chem 269 (41): 25277