CXCR4, NT (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)

HIV and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (10,11).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. \n\nRecommended Dilution: \nWestern Blot: 1-2ug/ml\nImmunoprecipitation: 10ug/ml\nImmunocytochemistry: 10ug/ml\nOptimal dilutions to be determined by researcher. \n\nPositive Control:\nHeLa whole cell lysate\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C8375

Size

100ug

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.01% sodium azide.

P Type

Pab

Purity

Purified IgG

Isotype

IgG

References

1. Dimitrov DS. Cell 1997;91:721-730\n2. Feng Y et al. Science 1996;272:872-7.\n3. Berson JF et al. J Virol 1996;70:6288-95.\n4. Doranz BJ et al. Cell 1996;85:1149-1158 \n5. Endres MJ et al. Cell 1996;87:745-756\n6. Bleul CC et al. Nature 1996;382:829-833\n7. Oberlin E et al. Nature 1996;382:833-835\n8. Lapham CK et al. Science 1996;274:602-5.\n9. Leoetscher M et al. J Biol Chem 1994;269:232-237\n10. Brelot A et al. J Virol 1997;71:4744--954751.\n11. Lu Z et al. Proc Natl Acad Sci USA 1997;94:6426-6431

Additional Info

Recognizes human CXCR4. Species crossreactivity: mouse.