CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60kD member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7Ra precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein thatis 374(aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over (aa1-57), mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.
Applications:
Suitable for use in Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 2.5ug/10e6 cells. Detected CXCR5 in mouse splenocytes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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