CXCR5 is a 55-60kD member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7Ralpha+ precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57aa N-terminal extracellular region plus a 47aa C-terminal cytoplasmic domain. Over aa1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.
Applications:
Suitable for use in Flow Cytometry, Direct ELISA and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 2.5ug/10e6 cells. Detects CXCR5 in B220+ mouse splenocytes.
Immunocytochemistry: 8-25ug/ml. CXCR5 was detected in immersion fixed mouse splenocytes and immersion fixed A20 mouse B cell lymphoma cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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