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Cyclin A

Cyclin A

Cat no: C8452-08H

Supplier: United States Biological
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Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C8452-08H
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ul
Form: serum with 0.01% sodium azide
P type: Pab
Isotype: IgG
Purity: Serum
References: Wang J et al. Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma. Nature 343:555-7 (1990). Pines J & Hunter T Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature 346:760-3 (1990). Giordano A et al. A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells. Cell 58:981-90 (1989).
Additional info: Antiserum will specifically react with a 60kD Cyclin A protein. No reaction was observed against other related cyclins. Species Crossreactivity: Crossreacts with Human, Rat and Mouse.

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