Cyclin B1, CT (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)

In mammals, cyclin B associates with inactive p34, cdc2 Thr which facilitates phosphorylation of p34 at aa14 and Tyr15. This maintains the inactive state until the end of G2- cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, phase. The inactive cyclin B-p34 dephosphorylates aa 14 Thr Tyr cdc2 and 15 of p34 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin- dependent and is necessary for the cell to exit mitosis. So, cdc2 cyclin B-p34 plays a critical role in G2 to M transition.\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:200 for 10min at RT\nStaining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat cells, breast carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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