In mammals, cyclin B associates with inactive p34, cdc2 Thr which facilitates phosphorylation of p34 at aa14 and Tyr15. This maintains the inactive state until the end of G2- cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, phase. The inactive cyclin B-p34 dephosphorylates aa 14 Thr Tyr cdc2 and 15 of p34 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin- dependent and is necessary for the cell to exit mitosis. So, cdc2 cyclin B-p34 plays a critical role in G2 to M transition.
Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:200 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive Control:
Jurkat cells, breast carcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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