Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1) (BSA & Azide Free)

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipatation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2 hrs at RT\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cells. Tonsil or breast carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8453-10X

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8453-10.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Baroja A; et al., Cell Proliferation, 1996 29(2): 89-100. 2. Brandeis M; et al., Embo Journal, 1996 15(19): 5280-9. 3. David-Pfeuty T; et al., Oncogene, 1996 13(7): 1447-60. 4. Joyce NC;et al., Investigative Ophthalmology and Visual Science, 1996 37(4): 645-55. 5. Kakino S;et al., Cytometry, 1996, 24(1): 49-54. 6. Klotzbucher A; et al., Embo Journal, 1996, 15(12): 3053-64. 7. Maity A; et al., Oncogene, 1996 13(8): 1647-57. 8. Villa R; et al., International Journal of Cancer, 1996 66(1): 104-9. 9. Bernhard EJ; et al., Radiation and Environmental Biophysics, 1995 34(2): 79-83. 10. Girard F; et al., J of Cell Sci, 1995 108:2599-608.

Additional Info

Recognizes hamster Cyclin B1 at ~62kDa. \nSpecies Crossreactivity: human, mouse and rat