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Cyclin D1 (bcl 1)

Cat no: C8454-05M

Cyclin D1 (bcl 1)

Cyclin D1or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative protooncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL) \n\nMW of Antigen: 36kD \n\nEpitope: Not determined\n\nApplications and Recommended Dilutions: \nImmunoprecipitation (Co-precipitates Cdk4) (Native only) (Use Protein A) (Ab at 2ug/mg protein lysate) \nKinase Assay The optimal dilution for a specific application should be determined by the investigator. \n\nPositive Control: MAD109 cells. \n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8454-05M

Size

250ul

Applications

IP

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8454-05MX.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Lukas J, et. al. Molecular and Cellular Biology, 1995, 15(5):2600

Additional Info

Highly specific to cyclin D1 and shows no crossreactivity with cyclin D2 or D3. It supports the cdks-associated kinase activity Species Crossreactivity: human, mouse, rat.

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