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Cyclin D1, NT (CCND 1, CCND1, CCND1 Protein, B-cell CLL/lymphoma 1, B cell Leukemia 1, BCL1, BCL-1 Oncogene, D11S287E, G1/S-specific Cyclin D1, Parathyroid Adenomatosis 1, PRAD1, PRAD1 Oncogene, U21B31)

Cat no: C8454-01V

Cyclin D1, NT (CCND 1, CCND1, CCND1 Protein, B-cell CLL/lymphoma 1, B cell Leukemia 1, BCL1, BCL-1 Oncogene, D11S287E, G1/S-specific Cyclin D1, Parathyroid Adenomatosis 1, PRAD1, PRAD1 Oncogene, U21B31)

Regulated progression through the cell cycle requires sequential expression of a family of proteins called cyclins. Upon their induction, cyclins form complexes with specific cyclin-dependent kinases (CDKs), creating active holoenzymes that phosphorylate target proteins that are required for cell-cycle progression. Induction of the proto-oncogene cyclin D1, and its binding to CDK4 or CDK6, is a rate-limiting event during cell-cycle progression through G1 phase. Some studies suggested that cyclin D1 also has CDK-independent functions. Studies in zebrafish model showed that cyclin D1 is upregulated by meis1, and it controls several development processes and in some way, carcinogenesis.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: C8454-01X\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C8454-01V

Size

250ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fu, M., et al. (2004). Endocrinology 145, 5439-5447. 2. Bessa, J. et al. (2008).Development 135, 799-803

Additional Info

Recognizes zebrafish Cyclin D1.

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