Cyclin D1 (S90) (CCND1 Protein, Cyclin D1, Isoform CRA_c, cDNA, FLJ93625, Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1),mRNA, Cyclin D1, PRAD1: Parathyroid Adenomatosis 1, CCND1) (PE)

CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. It has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:50-1:100\nImmunohistochemistry: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C8454-01S-PE

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.He,Y.Y., Cancer Res. 68(10), 3752-3758 (2008). 2.Marsit,C.J., Clin. Cancer Res. 14(8), 2371-2377 (2008). 3.Caldon,C.E., Cancer Res. 68(8), 3026-3036 (2008).

Additional Info

Recognizes human Cyclin D1. Species Crossreactivity: mouse.