Early screening of a drug candidate's potential to induce or inhibit CYP1A1/2 is a critical step in the drug development process. Cayman's CYP1A1/2 Induction STEP Reporter Assay Kit (Luminescence) is a novel method to assess CYP1A2 induction. STEP (Surface Transfection and Expression Protocol) is a patented, solid phase transient transfection technology that overcomes many of the disadvantages of other transfection approaches. Cayman's assay consists of a 96-well plate coated with a STEP complex containing a Secreted Alkaline Phosphatase (SEAP) reporter regulated by the human CYP1A1/2 gene promoter. Cells grown on the CYP1A1/2 STEP Strip Plate will introduce the reporter gene. Binding of inducer-activated endogenous transcription factors to the CYP1A1/2 promoter initiates a signal resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of medium are removed at time intervals and SEAP activity is measured by adding a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high-throughput screening for potential CYP1A1 and CYP1A2 inducers.
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