Cytokeratin 18

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Paraffin): Antigen retrieval required\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-19S

Size

100ug

Applications

ELISA, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH7.4, 15mM sodium azide

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Taylor-Papadimitriou J, Stampfer M, Bartek J, Lewis A, Boshell M, Lane EB, Leigh I.M.: Keratin expression in human mammary epithelial cells cultured from normal malignanttissue: relation to in vivo phenotypes influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13.Kovarik J. et al.: J. Tumor Marker Oncol. 5, 219 (1990). 2. Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B.: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. 3. Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J.: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl.1988;3:50-5.

Additional Info

Recognizes human Cytokeratin 18 at ~45kD.

Alternative Names

CK 18, CYK18, Cytokeratin endo B, Keratin 18, Keratin D, Keratin type I cytoskeletal 18, Kerd, KRT18