Cytokeratin 5,6

The cytokeratins are a family of intermediate filaments fond in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen's disease. It does not react with pulmonary adenocarcinoma.\n\nApplications: \nSuitable for use in Immunohistochemistry, Western Blot, Immunofluorescence. Other applications not tested.\n\nRecommended Dilution: \nImmunohistochemistry (frozen or paraffin sections): 1:50-1:100\nWestern Blot: Recognizes a 58kD and 56kD band corresponding to cytokeratin 5 and 6. Cytoskeletal preparations of epidermis.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-37B

Size

50ug

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Mikaelian, I., et al.: Antibodies That Label Paraffin-Embedded Mouse Tissues: A Collaborative Endeavor. Toxicology Pathology 32: 181-191 (2004). 2. EQAS 2002 Evaluation, A. Dodson, ed., UK-NEQAS (2002). Immunocytochemistry Journal 1(3). 3. Clover, J., et al., Histopathology 31: 140-143 (1997). 4. Lobeck, H., et al.: Generation and characterisation of monoclonal antibodies to cytokeratins No. 4 and 5. Europ. Symp. Biol. Cytoskeleton, Helsinki, 18-21, 6, 89 (1989). 5. Mischke, D., Wild, G., J. Invest. Dermatology 88: 191 (1987). 6. Wild, G., Mischke, D., Exp. Cell Res. 162: 114 (1986).\n\n\n

Additional Info

Recognizes human cytokeratin 5. Demonstrates crossreactivity with cytokeratin 6. Demonstrates weak crossreactivity with cytokeratin 4 on immunoblots. Does not crossreact with cytokeratins 1, 7, 8, 10, 13, 14, 18 or 19. Recognizes basal cells and a part of stratum spinosum in the normal pavement epithelium.