Cytokeratin, pan

Cytokeratin filaments consist of a complex family of approximately 20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells. This antibody may be useful in determining if a neoplasm originated from an epithelial or non-epithelial source, and is especially useful for the distinction of metastatic malignant tumors of epithelial origin.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nWestern Blot: 1:2000\nImmunohistochemistry (Frozen): 1:100. Incubate for 1hr at RT.\nImmunohistochemistry (Paraffin): 1:50. Incubate for > 1hr at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-48B

Size

100ul

Applications

ICC, IHC, WB

Reactivities

Hum, Mouse

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Akat, K., et al., Acta Neuropathol. 106: 337-347 (2003). 2. Boehnke, K., et al., Eur. J. Cell Biol. 86: 731-746 (2007).

Additional Info

Recognizes most acidic (K1, K4, K8) and basic (K14) human cytokeratins (Mr 40,000-Mr 68,000 polypeptides). \nTumors Specifically Detected: All types of epithelia and epithelium-derived neoplasms. Species Crossreactivity: mouse