Cytokeratin, Pan

Cytokeratin filaments consist of a complex family of approximately 20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells. This antibody may be useful in determining if a neoplasm originated from an epithelial or non-epithelial source, and is especially useful for the distinction of metastatic malignant tumors of epithelial origin.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections. Requires epitope retrieval using citrate buffer, pH 6.0, or enzyme digestion (pepsin).\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nAdenocarcinoma or Skin or Squamous carcinoma\n\nExpected Staining Pattern: \nCytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-48D

Size

1ml

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified.

Isotype

IgG

References

1. Moll, R., et al., Cell 31: 11-24 (1982). 2. Moll, R., et al., Am. J. Pathol. 140(2): 427-447 (1992). 3. Steinert, P.M., et al., Cell 42: 411-420 (1985). 4. Sun, T.T., et al., J. Invest. Dermatol. 81(1 Suppl.): 109s-115s (1983). 5. Clausen, H., et al., J. Invest. Dermatol. 86(3): 249-254 (1986). 6. Pinkus, G.S., et al., Am. J. Clin. Pathol. 85(3): 267-277 (1986).

Additional Info

Reacts with different kinds of epithelium in human tissues, including epithelial cells from skin, lung, esophagus, duodenum, pancreas, bile duct and kidney. Also reacts with adenocarcinomas\nand squamous cell carcinomas.