Cytomegalovirus, Human, IgG, BioAssay(TM) ELISA Kit (CMV)

The United States Biological Cytomegalovirus, Human, IgG, BioAssay(TM) ELISA Kit is intended for use in evaluating serologic status to cytomegalovirus (CMV) infection.\n\nCytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. About half of pregnant women who contract a primary infection spread the disease to their fetus. When acquired in-utero, the infection may cause mental retardation, blindness, and/or deafness.\n\nSerological tests for detecting the presence of antibody to CMV can provide valuable information regarding the history of previous infection, diagnosis of active or recent infection, as well as in screening blood for transfusions in newborns and immuno-compromised recipients. United States Biological ELISA CMV IgG is an accurate serologic method to detect CMV antibody for identification of CMV infection.\n\nPurified CMV antigen is coated on the surface of microwells. Diluted serum is added to wells, and the CMV IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.\n\nSample size: 5ul\n\nSpecificity: 98%\n\nSensitivity: 97%\n\nTotal Time: ~75 minutes\n \nKit Components:\nC9098-03B: Microtiter Plate, 1x96 wells\nC9098-03C: Enzyme Conjugate, 1x12ml\nC9098-03D: Negative Calibrator (0IU/ml), 1x150ul\nC9098-0E: Cut-off Calibrator (1.2IU/ml) 1x150ul\nC9098-03F: Positive Calibrator I (6IU/ml), 1x150ul \nC9098-03H: Positive Calibrator II (18IU/ml), 1x150ul \nC9098-03J: Negative Control (<1IU/ml), 1x150ul\nC9098-03K: Positive Control (1.2-18IU/ml), 1x150ul\nC9097-03L: Wash Buffer,10x, 1x100ml\nC9097-03M: Sample Diluent, 1x22ml \nC9097-03N: TMB, 1x12ml \nC9097-03P: Stop Solution (2N HCl), 1x12ml\n \nStorage and Stability: \nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9098-03

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Voler, A., J.E. Bidwell, et al. Manual of clinical immunology. Chapter 69. 2. Rose, N. and Friedman, H. eds. Am. Soc. Microbiol. p.506, 1985. 2. Cremer, N.E. Antibodies in serodiagnosis of viral infection. p. 73. In Lennett E.H. ed. Laboratory diagnosis of viral infection. Mercel Dekker, Inc., New York, 1985. 3. Starr, S.E. and H.M. Friedman. \"Human CMV.\" Chapter 65. In Manual of Clin. Microbiol., 4th ed., Lennett, E.H. et al ed. Am. Soc. Microbiol. pp. 771-719, 1985.