Cytomegalovirus IgG (CMV) BioAssay(TM) ELISA Kit

Cytomegalovirus (CMV) is a member of the herpes group of viruses. Most adults and children who catch CMV have no symptoms and are not harmed by the virus. CMV infection is of clinical significance primarily in pregnant women, newborn infants with possible congenital infection, immunosuppressed transplant patients and individuals with AIDS. CMV is so prevalent as over 60% of people catch the infection at some time in their lives. Significant increases in CMV IgG antibody by ELISA suggest recent infection or reactivation of a latent CMV infection. ELISA can detect CMV IgM antibody in both primary CMV infections (93-100%) and in reactivated infection (40%). An IgM response may be reduced or absent in immunocompromised patients with active infection. In transplant patients the CMV infection can be associated with higher morbidity and mortality.\n\nSample Type: \nSerum/Plasma\n\nIntended Use: \nDetection of IgG antibody to Cytomegalovirus (CMV) in human serum or plasma.\n\nTest Principle: \nDiluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.\n\nKit Components:\nMicrowells coated with CMV antigen 1x12x8\nSample Diluent: 1 bottle (ready to use) 22ml\nCalibrator: 1 Vial (ready to use) 1.5ml\nPositive Control: 1 vial (ready to use) 1.5ml\nNegative Control: 1 vial (ready to use) 1.5ml\nEnzyme conjugate: 1 bottle (ready to use) 12ml\nTMB Substrate: 1 bottle (ready to use) 12ml\nStop Solution: 1 bottle (ready to use) 12ml\nWash concentrate 20X: 1 bottle 25ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9098-03G

Size

1Kit

References

1. Mangano WE; Gruninger RP. Use of viral cultures and serologic tests for cytomegalovirus infection.\nRational or random? Am J Clin Pathol 1996;106(2):180-4. 2. Gupta CK; Leszczynski J; Gupta RK; Siber GR. An enzyme immunoassay based microneutralization test for titration of antibodies to human cytomegalovirus (CMV) and its correlation with direct ELISA measuring CMV IgG antibodies. Biologicals 1996;24(1):41-9. 3. Guti