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Cytotoxic Cells, Nonspecific

Cat no: C9127

Cytotoxic Cells, Nonspecific

Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32kD membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals. \n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Inhibition Assay. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 10ug/ml; Detects a 32kD protein representing nonspecific cytotoxic cell receptor protein. \nInhibition Assay: Has been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures. \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9127

Size

200ug

Applications

ELISA, FC, IP, WB

Hosts

Mouse

Reactivities

Bov, Can, Prc

Form

Supplied in a liquid buffer with 1mg/ml BSA and 0.05% sodium azide. \n

P Type

Mab

Purity

Purified IgM

Isotype

IgM

Additional Info

Recognizes NCC. Crossreacts with Bovine, Canine, Equine, Porcine, Rat, Tilapia and Catfish.

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