DARPP32, phosphorylated (Thr34) (Dopamine- and cAMP-regulated Neuronal Phosphoprotein, DARPP-32, FLJ20940, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B)

DARPP-32 (dopamine and cyclic AMPregulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/\nthreonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological\nand behavioral phenotype (3).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1075-03D

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155. (2) Bibb, J.A. et al. (1999) Nature 402, 669-671.\n(3) Fienberg, A.A. et al. (1998) Science 281, 838-842.

Additional Info

Recognizes endogenous levels of DARPP-32, phosphorylated at (Thr34). Species Crossreactivity: mouse, rat.

Alternative Names

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B