Home  >  Products  >  DARPP32, phosphorylated (Thr75) (DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Protein Phosphatase 1 Regulatory Subunit 1B, PPP1R1B)
DARPP32, phosphorylated (Thr75) (DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Protein Phosphatase 1 Regulatory Subunit 1B, PPP1R1B)

DARPP32, phosphorylated (Thr75) (DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Protein Phosphatase 1 Regulatory Subunit 1B, PPP1R1B)

Cat no: D1075-05C

Supplier: United States Biological
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DARPP-32, a dopamine (DA) and cAMP-regulated ~32kD phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac(R). Applications: Suitable for use in Western Blot.. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Okadaic incubated rat caduate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D1075-05C
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES (pH 7.5), 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B
References: 1. Svenningsson P., E.T. Tzavara, R. Carruthers, I. Rachleff, S. Wattler, M. Nehls, D.L. McKinzie, A.A. Fienberg, G.G. Nomiko and P. Greengard. 2003. Diverse psychotomimetics act through a common signaling pathway. Science 302:1412-1415. 2. Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G. Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London) 418 (2002) 774 - 778. 3. Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A. and Morrisett, R.A.
Additional info: Recognizes human DARPP32, phosphorylated at (Thr75). Species Crossreactivity: mouse and rat.

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