DDAH2 (Dimethylarginine dimethylaminohydrolase II, NG30, G6a, OTTHUMP00000174406, OTTHUMP00000029307, DADB-110M10.5, OTTHUMP00000062666, NG-dimethylarginine dimethylamino hydrolase homolog, Dimethylarginine dimethylaminohydrolase II, DDAHII, NG30, G6a, DD

DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1560-01C1

Size

100ug

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Pasini AF, et al. Nebivolol treatment reduces serum levels of asymmetric dimethylarginine and improves endothelial dysfunction in essential hypertensive patients. Am J Hypertens. 2008 Nov;21(11):1251-7. PMID: 18772860 2. Imamura M, et al. Alterations of NOS, arginase, and DDAH protein expression in rabbit cavernous tissue after administration of cigarette smoke extract. Am J Physiol Regul Integr Comp Physiol. 2007 Nov; 293(5): R2081-9. PMID: 17881617 3. Hasegawa K, et al. Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2.Circ Res. 2007 Jul 20; 101 (2): e2-10. PMID: 17601800 4. Wakino S, et al. Pioglitazone lowers systemic asymmetric dimethylarginine by inducing dimethylarginine dimethylaminohydrolase in rats. Hypertens Res. 2005 Mar; 28 (3): 255-62. PMID: 16097370 5. Chen Y, et al. Dimethylarginine dimethylaminohydrolase and endothelial dysfunction in failing hearts. Am J Physiol Heart Circ Physiol. 2005 Nov; 289(5): H2212-9. PMID: 16024577

Additional Info

Recognizes human DDAH2. Species Crossreactivity: Mouse.