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DDX6 (DDX-6, DEAD-box protein 6, DEAD (Asp-Glu-Ala-Asp))

DDX6 (DDX-6, DEAD-box protein 6, DEAD (Asp-Glu-Ala-Asp))

Cat no: D1661-04

Supplier: United States Biological
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This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 0.1-0.3ug/ml ~ 50kD band observed in lysates of cell line Jurkat (calculated MW of 53.2kD according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55kD is observed. This band is not observed in the non-transfected HEK293. The endogenous 53kD protein is observed in both. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D1661-04
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
Alternative names: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), DEAD box-6, RCK, P54, HLR2, DDX6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD-box protien 6
References: 1. Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol. 2004 Apr;165(1):31-40. Epub 2004 Apr 5.
Additional info: Recognizes human DDX6. Species sequence homology: mouse, rat, canine.

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