Dectin-1 (C-type lectin domain family 7 member A, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Beta-glucan receptor, C-type lectin superfamily member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082) (APC)

Dectin-1 is a 43kD type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner. Mature mouse Dectin-1 is a 244aa glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain. The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25%-34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region. Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells. It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS. The CRD selectively binds b-glucan polymers, a major component of yeast and mycobacterial cell walls. Yeast b-glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast. Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast. It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling. Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling. Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels up to 1x10e6 cells or 50ul whole packed blood.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at 12 months at 4 degrees C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

D1876-48E

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Kanazawa, N., 2007, J. Dermatol. Sci. 45:77. 2. Brown, G.D., 2006, Nat. Rev. Immunol. 6:33. 3. Ariizumi, K. et al., 2000, J. Biol. Chem. 275:20157. 4. Heinsbroek, S.E.M. et al., 2006, J. Immunol. 176:5513. 5. Taylor, P.R. et al., 2002, J. Immunol. 169:3876. 6. Willment, J.A. et al., 2003, J. Immunol. 171:4569. 7. Palma, A.S. et al., 2006, J. Biol. Chem. 281:5771. 8. Gantner, B.N. et al., 2005, EMBO J. 24:1277. 9. Gantner, B.N. et al., 2003, J. Exp. Med. 197:1107. 10. Kennedy, A.D. et al., 2007, Eur. J. Immunol. 37:467. 11. Brown, G.D. et al., 2003, J. Exp. Med. 197:1119. 12. Yadav, M. and J.S. Schorey, 2006, Blood 108:3168. 13. Suram, S. et al., 2006, J. Biol. Chem. 281:5506. 14. Meyer-Wentrup, F. et al., 2007, J. Immunol. 178:154. 15. Saijo, S. et al., 2007, Nat. Immunol. 8:39. 16. Taylor, P.R. et al., 2007, Nat. Immunol. 8:31.

Additional Info

Recognizes mouse Dectin-1.