Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Delta-like protein 1 (DLL1) is a 90-100kD type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23aa transmembrane segment and a 155aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37% and 54% aa sequence identity with DLL2, 3 and 4, respectively. A 60kD ECD fragment released by ADAM9, 12 or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).\n\nApplications:\nSuitable for use in ELISA, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 8-25ug/ml detects DLL1 in immersion fixed HepG2 human hepatocellular carcinoma cell line. \nImmunohistochemistry: 8-25ug/ml detects DLL1 in immersion fixed paraffin embedded human squamous cell carcinoma tissue sections.\nOptimal dilutions should be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D2727-75

Size

100ug

Applications

ELISA, ICC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 3. Karanu, F.N. et al. (2001) Blood 97:1960. 4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 6. Takahashi, Y. et al. (2003) Development 130:4259. 7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. 8. Kiernan, A.E. et al. (2005) Development 132:4353. 9. Schmitt, T.M. and J.C. Zuniga

Additional Info

Recognizes recombinant human DLL1 in direct ELISAs. Does not crossreact with recombinant human DLL3 or recombinant human DLL4.