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Demethylase (LSD-type) Activity Assay Kit

Cat no: 700400

Demethylase (LSD-type) Activity Assay Kit

Lysine demethylases containing amine oxidase domains, including the lysine-specific demethylases LSD1 and LSD2, specifically demethylate histone H3 at lysine 4 in a FAD-dependent reaction. Cayman's Demethylase (LSD-type) Activity Assay provides a convenient fluorescence-based method for assaying LSD-type demethylase activity from cell lysates or purified enzyme preparations. The assay is based on the production of formaldehyde during demethylation of lysine 4 on a peptide corresponding to the first 21 amino acids of the N-terminal tail of histone H3. Cyclization of formaldehyde and acetoacetanilide in the presence of ammonia gives a fluorescent product for quantitation.

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SPECIFICATIONS

Catalog Number

700400

Form

96 Well

P Type

Assay Kits|Demethylase

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

Cayman's Demethylase (LSD-type) Activity Assay provides a fluorescence-based method for assaying LSD-type demethylase activity. In this assay, formaldehyde is measured directly, eliminating the need for a coupled-enzyme reaction system. Formaldehyde, produced during demethylation of lysine 4 on a histone H3 peptide, reacts with the detection reagents provided in the kit to give a brightly fluorescent product. The assay is easy to use and can be completed in under two hours.

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