Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2, Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL, DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.\n \nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000 dilution. Human spleen tissue lysate or THP-1 cell lysate can be used as positive control and an approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.\nOptimal dilutions to be determined by the researcher.\n \nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3201-02A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified immunoglobulin

Isotype

IgG

References

1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8 2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6 3. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14 4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9 5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular cloning and characterization of human and murine DNase II. Gene 1998;215:281-9

Additional Info

Recognizes human DNase II.

Alternative Names

Deoxyribonuclease II alpha, DNase II alpha, \nDeoxyribonuclease II Lysosomal, DNase II Lysosomal