Home  >  Products  >  Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2, Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL, DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)
Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2, Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL, DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)

Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2, Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL, DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)

Cat no: D3201-02A

Supplier: United States Biological
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Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 dilution. Human spleen tissue lysate or THP-1 cell lysate can be used as positive control and an approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3201-02A
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified immunoglobulin
Alternative names: Deoxyribonuclease II alpha, DNase II alpha, Deoxyribonuclease II Lysosomal, DNase II Lysosomal
References: 1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8 2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6 3. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14 4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9 5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular cloning and characterization of human and murine DNase II. Gene 1998;215:281-9
Additional info: Recognizes human DNase II.

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