Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Peptide ELISA: 1:2,000.
Western Blot: 1-3ug/ml. Approx. 55kDa band observed in Human Skeletal Muscle lysates (calculated MW of 53.5kDa according to NP_001918.3). Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See D3221-02B-P. Antibody