Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.
D3221-52A localizes Dsc3 present in all living epidermal layers, in glandular duct cells, in basal matrix cells and outer root sheath of hair follicles, basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea, and thymic reticulum cells. All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109kD and 10kD polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT).
Applications:
Suitable for use in Western Blot and Immunohistochemistry (frozen, paraffin). Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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